Summary
Type: SPE
Application #: 305740
Catalog#: 730180 - SPE Cartridges, Chromabond, NH2, 6ml, PP, 500mg 30/pk
Detection:
Conditions: Column conditioning: 5 mL methanol, then 5 mL diethyl ether and at last 5 mL hexane
(Do not let run the column dry.)
Sample aspiration: 5 mL aliquot of the pretreated sample was placed on top of the column
Washing: 5 mL diethyl ether, then 5 mL chloroform – isopropanol (2:1, v/v)
Elution: 5 mL diethyl ether – acetic acid (98:2, v/v)
Evaporation: to dryness under reduced pressure
Reconstituion: 5 mL methanol
Authors: Fellermeier, M. et al.
Source: Eur. J. Biochem. 268, 1596–1604 (2001)